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primary antibodies against cd3  (Novus Biologicals)


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    Novus Biologicals primary antibodies against cd3
    (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − <t>CD3</t> + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
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    Images

    1) Product Images from "Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation"

    Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

    Journal: bioRxiv

    doi: 10.64898/2026.02.06.704418

    (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
    Figure Legend Snippet: (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Techniques Used: Immunohistochemistry, Control

    Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).
    Figure Legend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

    Techniques Used: Immunohistochemistry

    (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).
    Figure Legend Snippet: (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

    Techniques Used: Control, Immunohistochemical staining

    (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
    Figure Legend Snippet: (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Techniques Used: Control



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    Image Search Results


    (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: bioRxiv

    Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

    doi: 10.64898/2026.02.06.704418

    Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + Ly6G − NK1.1 − CD19 − CD3 + T cells in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of T cells calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of T cells per uterine horn. (D) Immunohistochemistry: detection of CD3 + T cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=5, 48 hr n=6, scale bar= 50µm). (E) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − CD19 − NK1.1 + NK cells in uterine tissue digests. (F) Bar plot, FC quantification: Relative abundance of NK cells calculated as a percentage of total CD45 + cells per uterine horn. (G) Bar plot, FC quantification: Absolute counts of NK cells per uterine horn. (H) Immunohistochemistry: detection of DBA-bound NK cells in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (I) Population restricted FC analysis to identify CD45 + Ly6G − CD3 − NK1.1 − CD19 + B cells in uterine tissue digests (J) Bar plot, FC quantification: Relative abundance of B cells calculated as a percentage of total CD45 + cells per uterine horn. (K) Bar plot, FC quantification: Absolute counts of B cells per uterine horn. (L) Immunohistochemistry: detection of B220 + B cells (black arrows) in uterine tissues 24- and 48 hr after progesterone withdrawal (representative images: 24 hr n=4, 48 hr n=4, scale bar= 50µm). (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

    Techniques: Immunohistochemistry, Control

    Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

    Journal: bioRxiv

    Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

    doi: 10.64898/2026.02.06.704418

    Figure Lengend Snippet: Immunohistochemistry: detection of (A) CD3+ T cells, (B) DBA-lectin+ NK cells and (C) B220+ B cells in uterine tissue sections at 0hr (prior to breakdown, n=4), 12hr (tissue breakdown, n=4-6), 24hr (tissue repair, n=4-5) and 48hr (tissue remodelling, n=4-6) hr after progesterone withdrawal (representative images, scale bar=500µm).

    Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

    Techniques: Immunohistochemistry

    (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

    Journal: bioRxiv

    Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

    doi: 10.64898/2026.02.06.704418

    Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 − Ly6C + MHCII − monocytes in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of monocytes calculated as a percentage of total CD45 + cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of monocytes per uterine horn. (D) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G − SiglecF − CD64 + Ly6C − F4/80 + MHCII + macrophages in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of macrophages calculated as a percentage of total CD45 + cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of macrophages per uterine horn. (G) Population restricted FC analysis of CD45 + CD3 − CD19 − NK1.1 − CD11b + Ly6G + SiglecF − neutrophils in uterine tissue digests. (H) Bar plot, FC quantification: Relative abundance of neutrophils calculated as a percentage of total CD45 + cells per uterine horn. (I) Bar plot, FC quantification: Absolute counts of neutrophils per uterine horn. (FC analysis groups: control (n=15), 0 hr (prior to breakdown, n=17), 12 hr (tissue breakdown, n=22), 24 hr (tissue repair, n=16) and 48 hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). (J-K) Immunohistochemical detection of CD14 + monocytes (cyan), CD64 + macrophages (yellow) and Ly6G + neutrophils (magenta) (merged and split channel) in uterine tissue cross sections at (J) 24 hr (repair, n=5) and (K) 48 hr (remodelling, n=3) following progesterone withdrawal (merged and split channel representative images, scale bar = 1000µm).

    Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

    Techniques: Control, Immunohistochemical staining

    (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: bioRxiv

    Article Title: Multimodal Profiling of Repair-associated Immune Dynamics in a Mouse Model of Menstruation

    doi: 10.64898/2026.02.06.704418

    Figure Lengend Snippet: (A) Population restricted FC analysis to identify CD45+ CD3- CD19- NK1.1- Ly6G- CD64- CD11c+ MHCII+ dendritic cells (DCs) in uterine tissue digests. (B) Bar plot, FC quantification: Relative abundance of DCs calculated as a percentage of total CD45+ cells per uterine horn. (C) Bar plot, FC quantification: Absolute counts of DCs per uterine horn. (D) Population restricted FC analysis to CD45+ CD3- CD19- NK1.1- CD11b+ Ly6G- SiglecF+ eosinophils in uterine tissue digests. (E) Bar plot, FC quantification: Relative abundance of eosinophils calculated as a percentage of total CD45+ cells per uterine horn. (F) Bar plot, FC quantification: Absolute counts of eosinophils per uterine horn. (FC analysis groups: control (n=15), 0hr (prior to breakdown, n=17), 12hr (tissue breakdown, n=22), 24hr (tissue repair, n=16) and 48hr (tissue remodelling, n=10) after progesterone withdrawal; statistical comparisons were made using Kruskal-Wallis tests with multiple comparisons. and * p< 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: Primary antibodies against CD3 (1:250 dilution; NB600-1441, Novus Biologicals), B220 (1:250 dilution; 14-0452-82, Invitrogen), CD14 (1:6000 dilution; ab221678, Abcam), CD64 (1:6000 dilution; 50086-R008, Sino Biological), Ly6G (1:10000 dilution; 127649, Biolegend) and DBA-lectin (1:1000 dilution; B-1035, Vector) were used in this study.

    Techniques: Control

    Fig. 2 SCLC-I Subtype exhibited a distinct immune microenvironment with higher CD8+ T cell infiltration. (A) Representative image of three distinct CD8+ T cell immune-phenotypes in ES-SCLC. (B) Pie chart showing the percentages of different CD8+ T cell immune-phenotypes(N = 93). (C) Comparison of molecular subtype composition across the three CD8+T cell immune-phenotypes, with the SCLC-I subtype most frequently associated with the “inflamed” phenotype. (D) SCLC-I subtypes had significantly more CD8+ T cells infiltration than the SCLC-A subtype (P = 0.0149). (E) Multiple immunofluorescence staining highlighting increased infiltration of CD3+CD8+ T cells in the SCLC-I subtype

    Journal: Journal of translational medicine

    Article Title: Immune profiling identifies CD161 + CD127 + CD8 + T cells as a predictive biomarker for Anti-PD-L1 therapy response in the SCLC-I subtype.

    doi: 10.1186/s12967-025-06724-8

    Figure Lengend Snippet: Fig. 2 SCLC-I Subtype exhibited a distinct immune microenvironment with higher CD8+ T cell infiltration. (A) Representative image of three distinct CD8+ T cell immune-phenotypes in ES-SCLC. (B) Pie chart showing the percentages of different CD8+ T cell immune-phenotypes(N = 93). (C) Comparison of molecular subtype composition across the three CD8+T cell immune-phenotypes, with the SCLC-I subtype most frequently associated with the “inflamed” phenotype. (D) SCLC-I subtypes had significantly more CD8+ T cells infiltration than the SCLC-A subtype (P = 0.0149). (E) Multiple immunofluorescence staining highlighting increased infiltration of CD3+CD8+ T cells in the SCLC-I subtype

    Article Snippet: Primary antibodies against CD3 (17617-1-AP, Proteintech; 1:1,000), CD8 (ab237709, Abcam; 1:2,000), CD161 (ab302564, Abcam; 1:300), CD127 (ab180521, Abcam; 1:50), PD-1 (ab234444, Abcam; 1:100), and CD4 (67786- 1-Ig, Proteintech; 1:800) were applied.

    Techniques: Comparison, Immunofluorescence, Staining